Fig. 6

Phosphorylation of PRP-treated hASCs. After incubation in serum-free DMEM for 6 h, cells were stimulated with PRP (1%) or PDGF-BB (10 ng/ml) for 30 min. Imatinib added for 1 h before stimulation with PRP. a Immunoblots of p-Akt and p-ERK1/2. b JNK activity examined in cell lysates, c-Jun as the substrate. Amounts of c-Jun quantitated in comparison with Ponceau S staining (lower panel). ERK extracellular signal-regulated kinase, Akt protein kinase B, PRP platelet-rich plasma, PDGF platelet-derived growth factor