Fig. 6

Adipogenic and osteogenic differentiation potential of hMuStem cells^HS and hMuStem cells^hPL. (a) Adipogenic and (b) osteogenic differentiation potential of hMuStem cells isolated and expanded with either HS or hPL (n = 3, donors #4–#6) evaluated at P4. a Intracytoplasmic lipid vesicles, visualized by Nile Red staining, quantified after 14 days in adipogenic induction medium. Nuclei counterstained with DAPI (blue). Staining quantified using AdipoRed™ assay. b After 21 days in specific induction medium, osteogenesis evaluated by Alizarin Red S staining (ARS) of calcium deposits. Staining quantified by optical density (O.D.) measurement after ARS dissolution. Scale bar, 100 μm. Donors #4, #5, and #6 represented by squares, triangles, and circles, respectively. Data presented as mean ± SD for each donor. HS human serum, hPL human platelet lysate, GM growth medium, RFU relative fluorescence units