Fig. 7

Influence of hPL on myogenic differentiation potential of hMuStem cells^HS. hMuStem cells^HS (n = 6, donors #7–#12) at P3–4 cultured for two passages with HS (hMuStem cells^HS/HS) or hPL (hMuStem cells^HS/hPL) and resulting myogenic pattern evaluated. Myogenic commitment assessed by determining (a) mRNA expression levels of MRFs MYF5, MYOD1, and DES, as well as (b) proportion of cells positive for each of these markers. Myogenic differentiation potential assessed by quantification of cells expressing (c) MyoD and (d) myogenin, and by determining (e) fusion index (FI), defined as percentage of nuclei identified in sarcomeric myosin heavy chain-positive (sMyHC⁺) myotubes. (f) Influence of hPL on contribution of hMuStem cells to myogenesis evaluated in coculture experiments with mouse D7 cells by quantification of human lamin A/C⁺ nuclei within hybrid sMyHC⁺ myotubes. Data presented as mean ± SEM (*p < 0.05, Wilcoxon matched-pairs signed rank test). HS human serum, hPL human platelet lysate