Fig. 8

Influence of hPL on adipogenic and osteogenic differentiation potential of hMuStem cells^HS. hMuStem cells^HS (n = 6, donors #7–#12) at P3–4 cultured for two passages with HS (hMuStem cells^HS/HS) or hPL (hMuStem cells^HS/hPL) and their potential to differentiate into adipogenic and osteogenic lineages in specific induction media evaluated. a, b Presence of intracytoplasmic lipid vesicles, visualized by Nile Red staining, assessed after 14 days in adipogenic induction medium. (a) Staining quantified using the AdipoRed™ assay and (b) mRNA levels of PPARγ quantified. c, d After 21 days in specific induction medium, osteogenesis evaluated by Alizarin Red S staining (ARS) of calcium deposits. (c) Staining quantified by optical density (O.D.) measurement after ARS dissolution and (d) mRNA levels of IBSP quantified. Data presented as mean ± SEM (*p < 0.05, Wilcoxon matched-pairs signed rank test). HS human serum, hPL human platelet lysate, RFU relative fluorescence units, GM growth medium, PPARγ peroxisome proliferator activated receptor gamma, IBSP integrin binding sialoprotein