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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Histone hypo-acetylation of Sox9 mediates nicotine-induced weak cartilage repair by suppressing BMSC chondrogenic differentiation

Fig. 3

Effects of nicotine on intracellular Ca2+ concentration and phosphatase activity of calcineurin in BMSCs. Assay was performed immediately (a and d) and 30 mins (b) after nicotine stimulus of in-vitro culture. a Change of fluorescence intensity of Ca2+ after the stimulus of nicotine at different concentrations. b Change of fluorescence intensity of calcineurin phosphatase activity of CaN calcineurin after the stimulus of nicotine at different concentrations. c Expression of the subtypes of nAChR in BMSCs. d Effects of MLA on nicotine’s augmentation of intracellular Ca2+ concentration in BMSCs. Data represent mean ± SEM (n = 3). *p < 0.05, **p < 0.01. Scale bar = 100 μm. BMSCs bone marrow mesenchymal stem cells, CaN calcineurin, MLA methyllycaconitine, nAChR nicotinic acetylcholine receptor,

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