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Table 1 Primer used for qPCR

From: Histone hypo-acetylation of Sox9 mediates nicotine-induced weak cartilage repair by suppressing BMSC chondrogenic differentiation

Gene

Forward Primer

Reverse Primer

Anneal

GAPDH

GCAAGTTCAACGGCACAG

GCCAGTAGACTCCACGACA

60 °C

Col2A1

GAGTGGAAGAGCGGAGACTACTG

CTCCATGTTGCAGAAGACTTTCA

60 °C

Sox9

CCAGCAAGAACAAGCCACAC

CTTGCCCAGAGTCTTGCTGA

60 °C

α3-nAChR

GGTGGATGACAAGACCAAAGC

AGGGCAGGTAGAAGACAAGCA

60 °C

α4-nAChR

CACGGTCTTCGTGCTCAATGT

CCTTGGTTGCAGATGTCACTC

62 °C

α5-nAChR

GCTGCGCTGCTCTTGATGGT

CGTATGTCCACGAGCCGAAT

60 °C

α7-nAChR

ACAATACTTCGCCAGCACCA

GGCATTTTGCCACCATCAGG

60 °C

β2-nAChR

GGAGTGGGAAGATTACCGCCTCA

AGTCGTCGTGGTTCTCGTTGCG

60 °C

β4-nAChR

GATTCTCCCAAGTCAGAACCTTT

AAGCTGGAGATTTGATGTGGTTA

60 °C

  1. Abbreviations: PCR polymerase chain reaction, GAPDH glyceraldehyde phosphate dehydrogenase, Col2A1 α1 chain of type II collagen gene, Sox9 SRY-type high mobility group box9, nAChR nicotinic acetylcholine receptor