Gene | Forward Primer | Reverse Primer | Anneal |
---|---|---|---|
GAPDH | GCAAGTTCAACGGCACAG | GCCAGTAGACTCCACGACA | 60 °C |
Col2A1 | GAGTGGAAGAGCGGAGACTACTG | CTCCATGTTGCAGAAGACTTTCA | 60 °C |
Sox9 | CCAGCAAGAACAAGCCACAC | CTTGCCCAGAGTCTTGCTGA | 60 °C |
α3-nAChR | GGTGGATGACAAGACCAAAGC | AGGGCAGGTAGAAGACAAGCA | 60 °C |
α4-nAChR | CACGGTCTTCGTGCTCAATGT | CCTTGGTTGCAGATGTCACTC | 62 °C |
α5-nAChR | GCTGCGCTGCTCTTGATGGT | CGTATGTCCACGAGCCGAAT | 60 °C |
α7-nAChR | ACAATACTTCGCCAGCACCA | GGCATTTTGCCACCATCAGG | 60 °C |
β2-nAChR | GGAGTGGGAAGATTACCGCCTCA | AGTCGTCGTGGTTCTCGTTGCG | 60 °C |
β4-nAChR | GATTCTCCCAAGTCAGAACCTTT | AAGCTGGAGATTTGATGTGGTTA | 60 °C |