Allogeneic transplantation of mobilized dental pulp stem cells with the mismatched dog leukocyte antigen type is safe and efficacious for total pulp regeneration

Table 2 Canine primers for real-time reverse transcription-polymerase chain reaction for immunomodulatory factors

Gene		5′–3′ DNA sequence	Accession number	Product (base pairs)
PTGES	Forward	GCCGCTGTGACTGTACC	NM_001122854	190
PTGES	Reverse	TGGTCCAATCAGCCACTTC	NM_001122854	190
COX-2	Forward	GTTCATTCCTGATCCCCAAG	NM_001003354	186
COX-2	Reverse	TTGAAAAGGCGCAGTTTATG	NM_001003354	186
IL-6	Forward	TCCAGAACAACTATGAGGGTGA	NM_001003301.1	l00
IL-6	Reverse	TCCTGATTCTTTACCTTGCTCTT	NM_001003301.1	l00
TGF-β	Forward	CTGGAGTCGTGAGGCAGTG	NM 0010033309.1	96
TGF-β	Reverse	GCAGTGTGTTATCTTTGCTGTCA	NM 0010033309.1	96
IDO-1	Forward	GGAAAGGCAACTCCAAACTG	XM_532793	124
IDO-1	Reverse	CCCAGCAGAATGTCAAAGC	XM_532793	124
β-actin	Forward	AAGTACCCCATTGAGCACGG	Z70044	257
β-actin	Reverse	ATCACGATGCCAGTGGTGCG	Z70044	257

PTGES prostaglandin E synthase, COX-2 cyclooxygenase-2, IL interleukin, TGF transforming growth factor, IDO-1 indoleamine 2,3-dioxygenase 1.

ISSN: 1757-6512