Fig. 2

Treatment with senolytic drugs did not support long-term expansion or affect molecular markers of senescence. Long-term growth curves of cumulative population doublings (cPDs) a after initial pulse treatment for 3 days (d; period indicated by dashed lines) or b with continuous treatment with drugs (results exemplarily depicted for one donor). c Relative telomere length measured as telomere to single copy gene (T/S) ratio (monochrome multiplex qPCR) of non-senescent and senescent MSCs with and without senolytic drug treatment (n = 3, mean ± SD; **p ≤ 0.01). d Comparison of real and predicted passage numbers in non-senescent and senescent MSCs with and without senolytic drug treatment. e Average number of predicted passages directly compared for the different treatments (n = 3, mean ± SD). NR nicotinamide riboside