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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions

Fig. 6

Mobilized human CD34+ bone marrow stem cells synthesize and release insulin in culture. Culture supernatants were analyzed by mass spectrometry. a Tracing of insulin alpha chain peptide GIVEIQCCTSICSL present in human mobilized CD34+ stem cell conditioned protein-free culture medium. b Tracing of insulin beta peptide VEALYLVCGER present in human mobilized CD34+ stem cell conditioned protein-free culture medium. c Tracing of insulin beta chain peptide EALYLVCGER present in human mobilized CD34+ stem cell conditioned protein-free culture medium. d Tracing of insulin beta peptide FVNQHLCGSHLVEALYLVCGER labeled with 13C-leucine present in human mobilized CD34+ stem cells cultured in 13C-leucine containing SILAC metabolic labeling medium. Each result shown is from a single experiment

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Stem Cell Research & Therapy

ISSN: 1757-6512