DNA topoisomerase IIβ stimulates neurite outgrowth in neural differentiated human mesenchymal stem cells through regulation of Rho-GTPases (RhoA/Rock2 pathway) and Nurr1 expression

Table 2 Primer sequences for reverse transcription quantitative polymerase chain reaction (RT-qPCR)

Primers	Forward	Reverse	Annealing Temperature
GAPDH	GCGAGATCCCTCCAAAATCAA	GTTCACACCCATGACGAACAT	60 °C
Topo IIβ	TTTTTCACCATCATTTGGTCTG	GGGCTTAGGGACTGTATCTGAA	60 °C
RhoA	CTGGTGATTGTTGGTGATGG	GCGATCATAATCTTCCTGCC	55 °C
Rac1	AACCAATGCATTTCCTGGAG	CAGATTCACCGGTTTTCCAT	60 °C
Cdc42	CTCCGGAAACTCAACCCAAA	GACGCAGAGGCTTTCAAACAG	60 °C
Rock2	TTGCTCTGGATGCAATACACTC	TCTCGCCCATAGAAACCATCA	55.5 °C
Nurr1	CTTGTGTTCAGGCGCAGTATG	GAGTGGTAACTGTAGCTCTGAGAAGC	60 °C

ISSN: 1757-6512