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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Co-stimulation of LPAR1 and S1PR1/3 increases the transplantation efficacy of human mesenchymal stem cells in drug-induced and alcoholic liver diseases

Fig. 6

Pre-incubation with LPA and/or S1P significantly enhances the therapeutic efficacy of transplanted hADMSCs in a murine National Institute on Alcohol Abuse and Alcoholism (NIAAA) model. a Representative images and corresponding NAS quantification of murine liver histology stained by H&E, Sirius Red, or human albumin antibody. Scale bar = 20 μm (n = 4). After the establishment of a NIAAA model with or without hADMSC administration (naive or LPA/S1P treated), changes in c hepatic human Down syndrome region; d serum ALT level, e serum AST level; f hepatic MDA content; g hepatic TNF-α protein level, h hepatic caspase-3/7 activity, i hepatic OSM mRNA level, j hepatic SREBP-1c protein level, k hepatic TGF-β protein level, l hepatic ALDH2 activity (n = 4), and m hepatic CYP2E1 protein expression were measured (n = 3). Data from each group are expressed as means ± SEM. Statistical comparison between groups was performed with the Kruskal–Wallis test followed by Dunn’s post-hoc test to detect differences in all groups. ***p < 0.001 versus healthy group; #p < 0.05 versus Gal/LPS group; ##p < 0.01 versus NIAAA group; ###p < 0.001 versus NIAAA group; @p < 0.05 versus NIAAA + LPA or S1P pre-treated stem cell transplantation group; @@p < 0.01 versus NIAAA + LPA or S1P pre-treated stem cell transplantation group; @@@p < 0.001 versus NIAAA + LPA or S1P pre-treated stem cell transplantation group. ALDH2, aldehyde dehydrogenase 2; ALT, alanine transaminase; AST, aspartate aminotransferase; CYP2E1, cytochrome P450 2E1; Gal, d-galactosamine; LPA, lysophosphatidic acid; LPS, lipopolysaccharides; MDA, malondialdehyde; OSM, oncostatin M; S1P, sphingosine-1-phosphate; SREBP, sterol regulatory element-binding protein; TGF, transforming growth factor; TNF, tumour necrosis factor

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