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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Long noncoding RNA H19 upregulates vascular endothelial growth factor A to enhance mesenchymal stem cells survival and angiogenic capacity by inhibiting miR-199a-5p

Fig. 1

Interference efficiency of H19 siRNA and expression of lncRNA-H19 in different conditions. a To evaluate interference efficiency of H19 siRNA, mRNA expression of lncRNA-H19 was detected using qRT-PCR. 1#, 2# and 3# represented H19-siRNA1#, H19-siRNA2# and H19-siRNA3# respectively. Transfection concentration of siRNA interference sequences were designed as 25 nM, 50 nM and 100 nM respectively. b qRT-PCR analysis of lncRNA-H19 expression in normoxia and H/SD conditions. MSCs+H19, MSCs transfected with lncRNA-H19; MSCs+H19 NC, MSCs transfected with lncRNA-H19 scramble; MSCs+si-H19, MSCs transfected with lncRNA-H19 siRNA; MSCs+si-H19 NC, MSCs transfected with lncRNA-H19 siRNA scramble. **P < 0.01 vs MSCs, ##P < 0.01vs MSCs+H19 NC, ††P < 0.01 vs MSCs+si-H19 NC. H/SD: hypoxia/serum deprivation, lncRNA-H19: long noncoding RNA H19, MSCs: mesenchymal stem cells, NC: scramble negative control

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