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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Long noncoding RNA H19 upregulates vascular endothelial growth factor A to enhance mesenchymal stem cells survival and angiogenic capacity by inhibiting miR-199a-5p

Fig. 5

LncRNA-H19 upregulated the expression of VEGFA. Alterations of VEGFA in different experimental groups were detected after lncRAN-H19 transfection or knockdown under normoxia and H/SD conditions. a, b Western blot analysis of the protein expression of VEGFA. c qRT-PCR analysis of the mRNA expression of VEGFA. MSCs+H19, MSCs transfected with lncRNA-H19; MSCs+H19 NC, MSCs transfected with lncRNA-H19 scramble; MSCs+si-H19, MSCs transfected with lncRNA-H19 siRNA; MSCs+si-H19 NC, MSCs transfected with lncRNA-H19 siRNA scramble. **P < 0.01 vs MSCs,## P < 0.01vs MSCs+H19 NC, †† P < 0.01 vs MSCs+si-H19 NC. H/SD: hypoxia/serum deprivation, MSCs: mesenchymal stem cells, NC: scramble negative control

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