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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Long noncoding RNA H19 upregulates vascular endothelial growth factor A to enhance mesenchymal stem cells survival and angiogenic capacity by inhibiting miR-199a-5p

Fig. 7

LncRNA-H19 targeted and negatively regulated miR-199a 5p. a Potential binding sites of lncRNA-H19 and miR-199a-5p. b Results of promega dual-luciferase reporter gene assay. Cells were transiently transfected with luciferase reporter contained wild-type (H19-WT) or mutant (H19-MU) H19. Measurement was performed with dual-luciferase reporter assay system, and luciferase activity was analyzed. Blank control, MSCs transfected with luciferase reporter containing H19-WT or H19-MU; miR-199a-5p, MSCs cotransfected with miR-199a-5p mimics and luciferase reporter containing H19-WT or H19-MU; miR-199a-5p NC, MSCs cotransfected with miR-199a-5p mimics negative control and luciferase reporter containing H19-WT or H19-MU. c Alterations of miR-199a-5p after lncRNA-H19 transfection or knockdown under normoxia and H/SD conditions. qRT-PCR were performed to explore expression of miR-199a-5p in different experimental groups under normoxia and H/SD conditions. MSCs+H19, MSCs transfected with lncRNA-H19; MSCs+H19 NC, MSCs transfected with lncRNA-H19 scramble; MSCs+si-H19, MSCs transfected with lncRNA-H19 siRNA; MSCs+si-H19 NC, MSCs transfected with lncRNA-H19 siRNA scramble. **P < 0.01 vs MSCs, ##P < 0.01vs MSCs+H19 NC, ††P < 0.01 vs MSCs+si-H19 NC. H/SD: hypoxia/serum deprivation, MSCs: mesenchymal stem cells, NC: scramble negative control

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