Fig. 2

Morphological cell changes during Neu-Dif. hADSCs isolated from healthy or ND subjects and differentiated for 37 days as indicated in Methods. Cell morphology at days 0 (MSCs), 21 (NSPs) and 37 (NLCs) of neural differentiation showing progeny of single cell-derived neurosphere and neural-like structures (a). Immunostaining assays of MSCs (at day < 0) and NLCs (at day 37) stained with TUJ1 (yellow) and nuclei counterstained with DAPI (blue) (b). Triple labeling of the single-cell suspension fully differentiated at D37 stained with TUJ1 (red), O4 (green) and GFAP (blue) (c). D day, DAPI 4′,6-diamidino-2-phenylindole, MSC mesenchymal stem cell, ND neurodegenerative disease, NLC neuron-like cell, NSP neurosphere-like structure