Fig. 4

ATP6AP2 signaling is dependent on Wnt(s) signaling in hADSCs. hADSCs isolated from healthy and ND subjects and differentiated for 37 days. Cell lysates (80–150 μg of protein) separated by SDS-PAGE and immunoblotted with antibodies raised against p-JNK, total JNK1 and β-catenin. Role of renin and canonical/noncanonical Wnt signaling evaluated. NLCs at day 37 treated after serum starvation overnight with human recombinant renin (hRenin, 50 nM) as indicated in Fig. 3. NLCs at day 36 treated overnight with Wnt3a (50 ng/ml, 24 h) or Wnt-antagonist DKK1 (200 ng/ml, 24 h) and Wnt5a (100 ng/ml, 24 h), and at day 37 with hRenin (50 nM, 0–1 h). Representative blots (a, b) and quantitative variations (c–f) shown. ^₤P < 0.01, Wnt5a-treated vs untreated cells. hRenin human recombinant renin, JNK Jun N-terminal kinase