Fig. 5

Transition of Wnt signaling responsiveness from Wnt/β-catenin to Wnt/planar cell polarity (PCP) during Neu-Dif. hADSCs cultured and differentiated as indicated in Methods; mRNA expression determined by RT-qPCR and normalized to GAPDH. Expression levels of canonical and noncanonical genes (β-catenin, LRP5 and LRP6 (a), Fzd-3, Fzd-6, PTK-7 and VANGL (b), Celsr(s) (c), Wnt(s) (d)), and caveolar/lipid raft key genes (CAVs (e), FLOTs (f)) and target gene of ATP6AP2 (VEGF/PEDF (g)) for healthy group. CAV-1/CAV-2 expression in ND group (h). *^§P < 0.05,**P < 0.001,***P < 0.0001: *D0/14/30/37 vs day < 0, ^§ND vs healthy. CAV caveolin, D day, FLOT flotillin, PEDF pigment epithelium-derived factor, VEGF vascular endothelial growth factor