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Table 3 Membrane fluidity assessment

From: Essential role of ATP6AP2 enrichment in caveolae/lipid raft microdomains for the induction of neuronal differentiation of stem cells

 

Fluorescence anisotropy

Percentage of control

P value

MSCs

 Control (untreated cells)

0.184 ± 0.012

100

 

 GW

0.215 ± 0.019

116.8

< 0.05

 nSMase

0.148 ± 0.008

80.4

< 0.01

 siAT6AP2

0.219 ± 0.010

119.0

< 0.01

NLCs

 Control (untreated cells)

0.167 ± 0.009

100

 

 GW

0.208 ± 0.011

124.5

< 0.01

 nSMase

0.150 ± 0.018

89.8

NS

 siAT6AP2

0.203 ± 0.007

121.6

< 0.01

  1. The cells derived from adipose tissue of healthy subjects were labeled with 1,6-diphenyl-1,3,5-hexatriene, and the fluorescence anisotropy of the probe was determined. The measurements were performed at 37 °C for 2 min immediately after addition of the fluorescent probe. Results presented as mean ± SEM
  2. P < 0.05 and P < 0.001, treated cells compared with control cells
  3. GW GW4869, MSC mesenchymal stem cell, NLC neural-like cell, NS not significant