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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience

Fig. 2

Morphological changes induced by PL (representative case). a When observed by inverted microscope (10×), MCSs cultivated in presence of PL appear smaller, more refractive and less attached to plastic support than MCSs cultured with FBS. b Morphological changes confirmed by flow cytometry analysis of FSC and SSC, as cells cultured in PL showed reduction of FSC and SSC rate representative of less complexity and smaller dimensions. DMEM Dulbecco’s modified Eagle medium, FBS fetal bovine serum, FSC forward scatter, MSC mesenchymal stromal/stem cell, PL platelet lysate, SSC side scatter

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