Fig. 4

Induction of T-regulatory cell population by MSCs cultured in PL or FBS-containing medium. Data presented as mean ± SD with n = 10. T_reg cell induction by MSCs evaluated as percentage of CD25^High/CD4⁺/CD127^Low/− PBMCs after 7 days of coculture. No significant differences found between MSCs cultured in PL or FBS-containing medium. In both culture conditions at day 7, percentage of CD4⁺/CD25^high/CD127^Low/− T cells was significantly higher (*p < 0.05) in cocultures with MSCs compared to PBMCs alone. DMEM Dulbecco’s modified Eagle medium, FBS fetal bovine serum, IL interleukin, MSC mesenchymal stromal/stem cell, PBMC peripheral blood mononuclear cell, PL platelet lysate