Skip to main content
Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience

Fig. 5

Inhibition of MLR by MSCs cultured in PL or FBS-containing medium. Data presented as mean ± SD with n = 10. Inhibition of PMBC responder (R) proliferation in presence of both allogeneic (PBMC stimulator (S)) and polyclonal (aCD3/28) stimuli evaluated as percentage of proliferation (% CFDA-SE-positive cells) in presence/absence of MSCs on 7-AAD CD45/CD3+ cells. No significant differences found between MSCs cultured in PL or FBS-containing medium. In both culture conditions, MSCs significantly reduce PBMC proliferation compared to PBMCs alone (*p < 0.05) in a dose-dependent manner. FBS fetal bovine serum, MSC mesenchymal stromal/stem cell, PL platelet lysate

Back to article page