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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The presence of human mesenchymal stem cells of renal origin in amniotic fluid increases with gestational time

Fig. 3

AFCs express typical MSC-associated proteins as well as CK19 and show multilineage differentiation potential in vitro. AFCs cultured in distinct media compositions stained positive for Vimentin and CD133 but negative for E-Cadherin. CK19, an established kidney epithelial marker, also expressed. Cell nuclei stained using Hoechst/DAPI (a). Flow cytometry-based analysis confirmed expression of MSC markers CD73, CD90 and CD105, and negativity for hematopoietic markers CD14, CD20, CD34 and CD45 (antibody isotype controls represented by thin lines; bold lines indicate histograms of distinct proteins) (b). Analysis of multilineage differentiation capacity of AF-MSCs revealed Alizarin Red staining of osteoid matrix-like structure in osteogenic medium, Alcian Blue staining of proteoglycans in chondrogenic media and lipid droplet formation around the cells in adipogenic media (c). HREpC human renal epithelial cell, RCM renal cell medium

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