Fig. 4

Derivation of clinical-grade hESCs by MTP under feeder-based and feeder-free conditions. To generate clinical-grade hESCs, mechanically isolated ICM initially cultured on GMP/clinical-grade extracellular matrix, such as rLaminin-521 or CELLstart, for a few days before being transferred onto GMP-grade HFFs (for feeder-based system) or fresh matrix-coated plates (for feeder-free system) to generate hESC colonies. ICM: inner cell mass, GMP: good manufacturing practice