High-efficiency derivation of human embryonic stem cell lines using a culture system with minimized trophoblast cell proliferation

Table 1 Efficiency of hESC derivation using WEC, MID, and MTP techniques^a

	Number of embryos^b	Outgrowth (day 3)	Number (%) of established hESC lines
WEC	3	3	0
MID	6	6	2 (33)
MTP	10	10	7 (70)

hESC human embryonic stem cell, WEC whole embryo culture, MID mechanical ICM dissection, ICM inner cell mass, MTP minimized trophoblast proliferation
^aExperiments presented were performed by the same personnel
^bAll embryos used were discarded embryos with genetic abnormalities, diagnosed by performing blastomere biopsy and preimplantation genetic diagnosis

ISSN: 1757-6512