Fig. 3

Measurement of cellular bioenergetics using XF24 Seahorse analyzer. Mesenchymal stem cells (MSCs) at passages 3, 5, and 7 were cocultured with leukocytes (with or without E06 blocking antibody) at a ratio of 1:10 (MSCs:leukocytes) for 72 h. a Basal respiration rate, b spare respiratory capacity, c coupling efficiency, and d maximal respiration rate were measured in MSCs. There were no significant changes observed in these parameters in MSCs with the increase in passage number, in the presence of leukocytes, and in the presence of E06 antibody. The values are normalized to cell number in each well. Data are represented as mean ± SD (n = 4–6). MSC + L,  MSCs + leukocytes; MSC + L + Ab,  MSCs + leukocytes + E06 antibody