Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Nitric oxide balances osteoblast and adipocyte lineage differentiation via the JNK/MAPK signaling pathway in periodontal ligament stem cells

Fig. 1

Nitric oxide (NO) and its synthesizing enzymes inducible NO synthase (iNOS) and endothelial NO synthase (eNOS) are expressed in periodontal ligament stem cells (PDLSCs). a,b Immunofluorescent staining and flow cytometry show that cells used in this study express CD146 and CD44 but not CD45. c–e Western blot, RT-PCR, immunofluorescent staining, and flow cytometry show that PDLSCs express iNOS and eNOS. Human umbilical vein endothelial cells (HUVECs) were used as positive control. Scale bar = 50 μm. f PDLSCs were treated with the NOS inhibitor l-NG-monomethyl arginine (l-NMMA) for 6 h. Western blotting shows that 1 mM l-NMMA significantly inhibits iNOS and eNOS expression. g NO was detectable in PDLSC culture supernatant and was significantly downregulated by l-NMMA and upregulated by sodium nitroprusside (SNP). nNOS, neuronal nitric oxide synthase

Back to article page