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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Nitric oxide balances osteoblast and adipocyte lineage differentiation via the JNK/MAPK signaling pathway in periodontal ligament stem cells

Fig. 2

NO has no influence on PDLSC proliferation and apoptosis, while physiological levels of NO are necessary for the mineralization of PDLSCs. a When NO generation was blocked by l-NG-monomethyl arginine (l-NMMA), there was no change in the proliferation capacity of PDLSCs, as evidenced by EdU assay. b Flow cytometric analysis shows that l-NMMA had no effect on PDLSC apoptosis. c Alizarin Red staining shows that l-NMMA (1 mM) treatment significantly reduced the formation of mineralized nodules in PDLSCs. Data are representative of three independent experiments. *P < 0.05, **P < 0.01. d Real-time RT-PCR showing that l-NMMA treatment downregulated the expression of runt-related transcription factor 2 (Runx2), osterix (OSX), and osteopontin (OPN) mRNA, while NO donor sodium nitroprusside (SNP) treatment largely rescued the expression of these osteogenic markers. All experiments are representative of three replicates. *P < 0.05, **P < 0.01

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