Fig. 3
The adipogenic capacity of PDLSCs is enhanced when endogenous NO production is blocked. a Four weeks after induction of adipogenic differentiation, PDLSCs were stained with Oil Red O. Data are representative of three independent experiments. Scale bar = 50 μm. b ImageJ semiquantitative analysis showing that l-NG-monomethyl arginine (l-NMMA) significantly induced cellular lipid accumulation. This effect was reversed by the NO donor sodium nitroprusside (SNP). **P < 0.01. c Real-time RT-PCR analysis of gene expression during adipogenic differentiation. Lipoprotein lipase (LPL), peroxisome proliferator-activated receptor (PPAR)γ, and CCAAT-enhancer binding protein (C/EBP)α mRNA levels were upregulated in PDLSCs treated with l-NMMA, and NO donor SNP reduced the upregulation of these genes. **P < 0.01