Fig. 4

Ratio of the sorted surface, stromal and perivasucular cells. a Scheme for isolating surface, stromal and perivasucular cells. Synovium was digested and filtrated for bulk cells. From the bulk cells, dead cells were removed, then hematopoietic and endothelial cells were removed for non-hematopoietic lineage cells. From the non-hematopoietic lineage cells, surface cells were sorted for CD55, stromal cells were negative sorted for CD55 and CD271, and perivascular cells were sorted for CD90 and CD271. Non-hematopoietic lineage cells consisted of PI⁻ CD31⁻ CD45⁻ CD235a⁻ cells. b Ratio of the gated cells to PI⁻ cells. Bar shows mean ± SD. The analyzed synovium were obtained from eight donors were analyzed. **p < 0.01 by Wilcoxon matched-pairs signed rank test. c Ratio of the sorted cells to non-hematopoietic lineage cells. Bar shows mean ± SD. Synovia derived from eight donors were analyzed. **p < 0.01 by the Friedman test followed by Steel-Dwass multiple comparisons