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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Allogeneic transplantation of programmable cells of monocytic origin (PCMO) improves angiogenesis and tissue recovery in critical limb ischemia (CLI): a translational approach

Fig. 3

Representative phase contrast pictures of tubular network on Matrigel formed by (a) PCMO hypoxic-conditioned and (b) PCMO non-hypoxic-conditioned cell culture supernatants derived from donor D1, D2, D3, D4, D5 and pooled donor samples. c Corresponding extracted skeletons of tubular networks identifying segments (yellow color), mesh (blue color) and nodes (red color). Branches are marked green (not analyzed). Quantitative assessment (d) was performed by the angiogenesis analyzer of the ImageJ software. (H hypoxic conditioned, N non-hypoxic conditioned; *p < 0.05 compared to D1N, D2N, D2H, D3N, D3H, D4N, D5N, D5H, pool/N)

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