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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo

Fig. 2

Monosodium urate (MSU) induced endothelial colony-forming cell (EFCF) necrosis. a ECFC were treated with MSU at the indicated concentration and the cell death was analyzed by fluoresce microscopy after 24 h (n = 5). b Kinetics of ECFC death were studied by fluorescence microscopy (n = 4). c Representative images of viable (V) and necrotic (N) cells are shown. Original magnification, 1200×. Scale bar = 10 μm. d The percentage of cell death was measured by fluorescence microscopy in the presence or absence of Z-VAD-fmk (30 μM). The percentage of positive cells for active caspase-3 (e), interleukin (IL)-1β levels (f), and reactive oxygen species (ROS) formation (g) were analyzed by flow cytometry after 24 h (n = 3). *p < 0.05 vs untreated. NS, not significant

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