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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo

Fig. 3

Preconditioned endothelial colony-forming cells (EFCF) are more resistant to DAMP- or TNFα-induced cell death. Nonpreconditioned or preconditioned ECFC (npECFC or pECFC, respectively) were incubated with monosodium urate (MSU), tumor necrosis factor (TNF)α, or histones at the indicated concentrations. a The nuclear morphology was analyzed by fluorescence microscopy and (b) the percentage of annexin V and propidium iodide (PI) double-positive cells was measured by flow cytometry after 24 h (n = 6). c ECFC were incubated with high glucose, TNFα, or their combination and cell death was analyzed after 24 h (n = 6). *p < 0.05 vs untreated npECFC; #p < 0.05 vs npECFC with the same treatment

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Stem Cell Research & Therapy

ISSN: 1757-6512