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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo

Fig. 4

Acidic preconditioning improved endothelial colony-forming cell (EFCF) proliferation and tubule formation under adverse conditions. Nonpreconditioned or preconditioned ECFC (npECFC or pECFC, respectively) were treated with high glucose, tumor necrosis factor (TNF)α, or their combination, and the different angiogenic responses were measured. a Cell counts were performed after 48 h using a Neubauer chamber (n = 4). b Cells were seeded on Geltrex™ matrix and the number of branch points per field was measured after 18 h. c Representative images of six different experiments. Original magnification, 40×. *p < 0.05 vs untreated npECFC; #p < 0.05 vs npECFC with the same treatment; &p < 0.05 vs TNFα or glucose alone

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Stem Cell Research & Therapy

ISSN: 1757-6512