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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo

Fig. 6

Acidic preconditioning enhanced endothelial colony-forming cell (EFCF)-mediated neovascularization in ischemic hind limbs of normoglycemic and diabetic mice. Phosphate-buffered saline (PBS), and nonpreconditioned or preconditioned ECFC (npECFC or pECFC, respectively) were infused intravenously in normoglycemic and type 2 diabetic (T2D) mice 5 h after ischemia-inducing surgery. a Perfusion index was calculated by Doppler analysis after 7 and 14 days postischemia. Representative images of T2D animals are shown. b Histologic analysis of gastrocnemius muscles, stained with hematoxylin and eosin (H/E) or Masson’s trichrome, was performed after 14 days postischemia. Original magnification, 400×. Scale bar = 80 μm c Inflammation score was calculated in H/E stained sections. d Vascular density was measured in Masson’s trichrome stained sections. n = 6 per group. *p < 0.05 vs PBS; #p < 0.05 vs npECFC; &p < 0.05 vs the same treatment in the normoglycemic group; ¥p < 0.05 vs no ischemia. AU, arbitrary units

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