Fig. 6

The effects of endometrial regenerative cells (ERCs) on regulatory B cells (Bregs) in vitro and the adoptive transfer of ERC-induced Bregs into colitis mice. Purified recipient B cells (10⁶/well) were stimulated with 10 μg/ml lipopolysaccharide (LPS) for 72 h. a CFSE-labeled B cells were harvested thereafter and analyzed by flow cytometry. The proliferation was expressed as a percentage of proliferative B cells versus untreated LPS-stimulated B cells. b The proportion of CD1d^hiCD5⁺ and c IL-10⁺ cells in CD19⁺ B cells was measured by flow cytometry. d Supernatants were harvested and IL-10 production was measured by ELISA. e The expression of IL-10 in CD1d^hiCD5⁺ and CD1d^lowCD5⁻ B cells from ERC-treated colitis mice. f Adoptive transfer of Bregs attenuated body weight loss and disease activity in colitis mice. Graphs represent mean ± SEM of triplicate experiments. P value was determined by one-way ANOVA. *P < 0.05. DSS, dextran sodium sulfate; PBS, phosphate-buffered saline