Fig. 3
From: Changes in phenotype and differentiation potential of human mesenchymal stem cells aging in vitro
Morphological evaluation of in-vitro aging MSCs. a Passaged MSCs were divided into three subpopulations, S1, S2, and S3, based on the forward (size) and side (granularity) scatter parameters in flow cytometry analysis. b, c Cell counts in the main subpopulation, S1, decreased by 64% and 24% from P3 to P8 in the DMEM (b) and αMEM (c) groups, respectively. n = 20; *p < 0.05, versus the corresponding P8-MSCs within the same subpopulation. αMEM, minimum essential medium alpha; DMEM, Dulbecco’s modified Eagle’s medium