Fig. 6
From: Changes in phenotype and differentiation potential of human mesenchymal stem cells aging in vitro
Adipogenic and osteogenic differentiation of MSCs derived from the DMEM-based expansion condition. MSCs at P4 (a, c) or P8 (b, d) were cultivated with either osteogenic differentiation media for 9 days or adipogenic differentiation media for 21 days. a, b Expression of osteogenic (Col I, RUNX2, ALP) and adipogenic (LPL, PPARγ) genes was determined by qPCR with GAPDH as the reference gene and nonconditioned MSCs at the corresponding passages as the control groups (red dashed lines). n = 4; **p < 0.01, ***p < 0.001, versus nonconditioned P4-MSCs or P8-MSCs. c, d Phase-contrast images of osteogenic and adipogenic samples were captured at the designated time points. White clusters in the adipogenic images represent lipids produced by the cells. Scale bars = 250 μm. ALP, alkaline phosphatase; Col I, type I collagen; LPL lipoprotein lipase; MSC, mesenchymal stem cell; P, passage; PPARγ, peroxisome proliferator-activated receptor γ; RUNX2, runt-related transcription factor 2