Fig. 1
From: Different effects of granulocyte colony-stimulating factor and erythropoietin on erythropoiesis
![Fig. 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13287-018-0877-2/MediaObjects/13287_2018_877_Fig1_HTML.gif)
Granulocyte colony-stimulating factor (G-CSF) treatment induced more R4 erythroblast cells than erythropoietin (EPO) treatment did in bone marrow (BM). a The experimental outline used to measure erythropoiesis status after G-CSF (n = 5) and EPO (n = 5) treatments in the BM is depicted. Saline-treated mice (n = 5) were used as negative controls. Flow cytometry analysis was performed 72 h after initial injection. The erythroblast cells were gated as R1 (CD71high, TER-119med), R2 (CD71high, TER-119high), R3 (CD71med, TER-119high), and R4 (CD71low or –, TER-119high) in all groups. Nonerythroid cells were gated as NE (TER-119 negative) (b). Cell numbers of nonerythroid (c) and total erythroid cells (sum of R1, R2, R3, and R4) (d), and the percentage of individual erythroblasts (R1, R2, R3, and R4) in the total number of erythroid cells in each group (e) were quantified. Data are reported as mean ± SD. *P < 0.05, **P < 0.01