Fig. 2

Granulocyte colony-stimulating factor (G-CSF) treatment induced more R4 erythroblast cells than erythropoietin (EPO) treatment did in the spleen. a The experimental outline used to measure the erythropoiesis status after G-CSF (n = 5) and EPO (n = 4) treatments in the spleen is depicted. Saline-treated mice (n = 5) were used as negative controls. Flow cytometry analysis was performed 72 h after initial injection. The erythroblast cells were gated as R1, R2, R3, and R4 in all groups (see Fig. 1 for details). Nonerythroid cells were gated as NE (b). Cell numbers of nonerythroid (c) and total erythroid cells (sum of R1, R2, R3, and R4) (d), and the percentage of individual erythroblasts (R1, R2, R3, and R4) in the total number of erythroid cells in each group (e) were quantified. Data are reported as mean ± SD. *P < 0.05, **P < 0.01