Fig. 3

Granulocyte colony-stimulating factor (G-CSF) treatment mobilized more newly synthesized reticulocytes than erythropoietin (EPO) treatment did. a The experimental outline for investigating erythrocyte mobilization in enhanced green fluorescent protein (EGFP) mice after G-CSF (n = 4) and EPO (n = 4) treatments is shown. Peripheral blood (PB) was collected 20, 40, and 60 h after initial injection. Flow cytometry analysis was performed to analyze the relative fold of EGFP⁺/TER-119⁺ cells (gated R1 region) compared with the before experiment (Before exp.) groups and the percentage of F22⁺ cells in EGFP⁺/TER-119⁺ cells at 20 h (b). Quantitative results of the relative fold of EGFP⁺/TER-119⁺ cells (c) and the percentage of F22⁺ cells in EGFP⁺/TER-119⁺ cells at 20, 40, and 60 h (d) are indicated. Data are reported as mean ± SD. *P < 0.05, **P < 0.01, versus the Before exp. groups; ^#P < 0.05