Fig. 5

Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) mostly differentiate into oligodendrocytes and astrocytes and a few neurons. a-d Oligodendrocytic differentiation of the transplanted mesenchymal stem cells (MSCs) was noted in the transplantation groups 8 weeks post-transplantation. e-h Astrocytic and i-l neuronal differentiation of the transplanted MSCs was also noted in the transplantation groups (red fluorescence for Olig1, glial fibrillary acidic protein (GFAP), and NeuN antibody, green fluorescence for hNuA antibody, blue for 4,6-diamino-2-phenyl indole (DAPI)). Arrows indicate a double-labelled cell. m Quantitative analysis of the Olig1⁺/hNuA⁺, GFAP⁺/hNuA⁺, and NeuN⁺/hNuA⁺ cell numbers in the single injection (SI), repeated injections at 3-day intervals (3RI) and repeated injections at 7-day intervals (7RI) groups. The results show that there was no significant difference in the differentiation ratio of human stem cells among the transplantation groups 8 weeks post transplantation. Scale bar = 50 μm