Fig. 6

Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) express Ki67 8 weeks following transplantation. Human nuclei antibody-positive cells (hNuA⁺), which are shown in green (b, f, j), were rarely associated with the cell cycle marker Ki67, which is shown in red (a, e, i); 4,6-diamino-2-phenyl indole (DAPI) counterstaining is shown in blue (c, g, k) in the single injection (SI) (a-d), repeated injections at 3-day intervals (3RI) (e-h) and repeated injections at 7-day intervals (7RI) (i-l) groups. Arrows indicate double-labelled cells. m Quantitative analysis of the Ki67⁺/hNuA⁺ cell numbers in the SI, 3RI and 7RI groups. The results show that the percentage of Ki67 in the 3RI group was greater than that in the SI group (**p < 0.01). Scale bar = 50 μm