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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media

Fig. 1

a Distinct stratified phases are clearly evident after 1 h of lipoaspirate treatment with collagenase solution at 37 °C. Stromal vascular fraction cells are contained in the lower clear phase; digested adipocytes as well as released oily lipids are stratified in the two upper phases. The image is representative of a completely accomplished digestion process. b Images of diluted stromal vascular fraction cells cytospun on a glass slide. Cells were differentially stained by May-Grunwald/Giemsa protocol. Scale bar = 10 μm

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