Fig. 1From: Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free mediaa Distinct stratified phases are clearly evident after 1 h of lipoaspirate treatment with collagenase solution at 37 °C. Stromal vascular fraction cells are contained in the lower clear phase; digested adipocytes as well as released oily lipids are stratified in the two upper phases. The image is representative of a completely accomplished digestion process. b Images of diluted stromal vascular fraction cells cytospun on a glass slide. Cells were differentially stained by May-Grunwald/Giemsa protocol. Scale bar = 10 μmBack to article page