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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media

Fig. 4

a Impact of different cryopreservation solutions on percent nucleated cell (NC) viability in thawed SVF products after 2 months of storage in liquid nitrogen. A, B, C and D are different cryopreservation solutions: solution A (10% Albital, 5% ACD-A, 10% DMSO, 75% saline solution), solution B (50% human serum, 5% Albital, 2.5% ACD-A, 10% DMSO, 32.5% saline solution), solution C (90% human serum, 10% DMSO), solution D (95% human serum, 5% DMSO). When compared with solutions C and D, the viability of NC stored for 2 months by solutions A and B was significantly lower. *p < 0.01, vs C and D; NSa, not significantly different vs C and vs Pre (one-way ANOVA for independent samples). b Impact of total NC concentration on SVF freezing on post-thaw cell viability. In the high NC concentration group (High), cell viability measured after thawing was significantly higher than in the low concentration (Low) group. §p < 0.05, vs High Group (Student’s t test for unpaired data). c Impact of longer term cryostorage on SVF samples. NC viability measured after 1 year of freezing was not significantly different when compared with results obtained after 2 months storage. *p < 0.01, vs Pre (one-way ANOVA for independent samples). NSb, not significantly different vs 2 months (one-way ANOVA for repeated measures). Tukey’s honestly different significance with Bonferroni’s correction as post-hoc test

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