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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media

Fig. 7

Representative images obtained from osteogenic, adipogenic, and chondrogenic differentiation assays performed on ASC after short-term or longer-term expansion at 1 × 103 cells/cm2 in the presence of 10% fetal bovine serum (FBS) or 5% supernatant rich in growth factors (SRGF) in the cell culture medium. The differentiation degree was quantified by image analysis of cell staining (adipogenesis and osteogenesis) or by morphometric analysis of spheroids (chondrogenesis); results are reported in histograms. The differentiation potential was shown to be not significantly affected when comparing ASC expanded in 10% FBS and in 5% SRGF-containing media, both at high and low passages. Scale bar = 100 μm. C.A., covered Area; MEM, minimum essential medium; Vol., volume; Unst., unstimulated

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