Fig. 8From: Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free mediaa Representative karyotypes of adipose tissue-derived stem cells (ASC) expanded at high passages in 10% fetal bovine serum (FBS)- or 5% supernatant rich in growth factors (SRGF)-containing medium. At least 20 metaphases were analyzed and no clonal or recurrent chromosomal alterations could be identified. b Displays images taken from colony formation assays in methylcellulose medium performed on high-passage ASC cultured in 5% SRGF- or 10% FBS-containing medium. ASC expanded utilizing both cell culture media failed to display colony formation. HT1080 fibrosarcoma cells were used as positive control (c+). Scale bar = 100 μm. MEM, minimum essential mediumBack to article page