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Table 1 Expression level of surface markers analyzed in adipose tissue-derived stem cells (ASC) present in the stromal vascular fraction (SVF) and in expanded ASC

From: Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media

Markers

Part A

Part B

ASC in SVF

Expanded ASC

Fresh

Thawed

10% FBS

5% SRGF

CD34

++

++

CD45

CD31

CD90

++

++

++

++

CD73

++

++

++

++

CD13

+

+

++

++

CD105

+−

+−

++

++

CD44

+

+

+

+

CD29

+−

+−

++

++

CD166

+−

+−

++

++

CD10

+

+

+

+

HLA I, ABC

+

+

+−

+−

HLA II, DR

CD106

+−

+−

CD36

CD146

+−

+−

CD235

CD144

CD11b

CD11c

CD14

  1. Part A reports the expression levels of selected markers in the 34++ 31SSChigh ASC contained in fresh or thawed SVF samples
  2. Part B reports the expression levels of the same markers in expanded ASC in the presence of 10% fetal bovine serum (FBS) or 5% supernatant rich in growth factors (SRGF) and alpha-minimal essential medium (α-MEM)
  3. Expression levels are described as + and – considering unlabeled cells as reference (see also Fig. 3): ++, bright expression; +, positive expression; +−, dim expression