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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models

Fig. 3

hES-AS protect MNs from oxidative stress. A Mouse motor neurons exposed in 96-well plates to 150 μM H2O2 for 6 h (bar 1) or left untreated (bar 4). During H2O2 treatment, neuron cultures supplemented with conditioned medium from hESC-derived astrocytes, differentiated for 28 days (ACM, bar 2), or with 20,000 of the same hES-AS (bar 3). After fixation, cells double-stained with anti-tubulin β3 antibody (neuron marker, green) and anti-Caspase-3a (apoptotic marker, red). Percentage of apoptotic neurons (Caspase3a over tubulin β3-positive cells) counted using high-content image screening system (Arrayscan; Cellomics). Results represent average ± SD for 10 wells of 96 well-plate per treatment (for each well, 49 fields were analyzed). *p < 0.05; **p < 0.01. b Left panel: representative image of neuron cultures with H2O2 treatment. Apoptotic neuronal cell bodies yellow (arrows, due to overlapping of red Caspase-3 staining with green tubulin β3). Right panel: with ACM, much less apoptotic yellow cells are seen. Scale bar: 100 μm. hESC human embryonic stem cell, H2O2 hydrogen peroxide

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