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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models

Fig. 4

hES-AS and their conditioned medium stimulate axonal outgrowth in cortical neurons. a Mouse cortical neurons cocultured with hES-AS (7-day differentiated APC) (2 × 104 and 4 × 104 cells), or with neurotrophin 3 (NT3) as positive control, or left untreated (negative control). Last row shows neurons cultured with conditioned medium from same hES-AS (taken after 48 h of culture). Representative images of cells stained with DAPI and by immunofluorescence for neurofilament-160 (NF160) and GFAP shown for each condition. Stimulation of axon and neurite outgrowth seen from NF160 stain and merge of NF160 (green) and GFAP (red). Scale bar = 100 μm b By high-content image screening analysis (Arrayscan; Cellomics), area covered by axon and neurite outgrowth quantified, using 49 fields for each of six replica wells from each experimental conditions. Error bars represent SD. *Student’s t test, p < 0.05). DAPI 4′,6-diamidino-2-phenylindole, GFAP Glial Fibrillary Acidic Protein

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