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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models

Fig. 7

hES-AS are post mitotic and maintain their astrocytic identity in vivo. ac High-content analysis of hES-AS cells in vitro displayed homogeneous expression of human GFAP (Stem123). %Ki67+ cells calculated as % Ki67+ nuclei / total number of nuclei. Ki67+ cells rarely found within hES-AS cell population (arrows). df Two million hES-AS injected intrathecally into the lumbar region twice, with interval of 21 days. Analysis of graft, 8 weeks post first cell injection, showed transplanted cells were located in subarachnoid space, attached to pia mater of lumbar spinal cord and nerve bundles. Cells maintained their astrocytic characters and homogeneously expressed human-origin GFAP. %Ki67+ hES-AS cells calculated as % Ki67+ nuclei / total number of nuclei of Stem123+ cells. Ki67+ staining very rare among hES-AS cells (arrows), indicating that cells are non-proliferative in vivo. hES-AS human embryonic stem cell-derived astrocytes (differentiated from APCs for 7 days), DAPI 4′,6-diamidino-2-phenylindole

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